Category

Genome Variant Analysis


Usage

java -jar GenomeAnalysisTK.jar -T QualifyMissingIntervals -R reference.fasta -I input.bam -o output.grp -L input.intervals -cds cds.intervals -targets targets.intervals


Manual

Argument name(s)Default valueSummary
Required Parameters
--targetsfile
 -targets
NAUndocumented option
Optional Outputs
--out
 -o
stdoutAn output file created by the walker. Will overwrite contents if file exists
Optional Parameters
--baitsfile
 -baits
NAUndocumented option
--coveragethreshold
 -cov
20minimum coverage to be considered sequenceable
--gcthreshold
 -gc
0.3upper and lower bound for an interval to be considered high/low GC content
--intervalsizethreshold
 -size
10minimum interval length to be considered
--mappingthreshold
 -mmq
20minimum mapping quality for it to be considered usable
--qualthreshold
 -mbq
20minimum base quality for it to be considered usable


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